2D cell culture systems have been standard for the past century, enabling findings in many applications, ranging from stem cell research, drug screening, and regenerative medicine. However, cells interact with neighboring cells, the extracellular matrix, and surrounding molecules in 3D. Cells in vivo experience an extremely complex three-dimensional environment at the organ, tissue and cellular level down and below the micrometer scale. The advances of Nanoscribe’s 3D Microfabrication have demonstrated how this technology enables the fabrication of intricate 3D microstructures such as 3D cell scaffolds. Now the company takes a further step forward and presents IP-Visio, the new printing material designed for the fabrication of biocompatible 3D microstructures.
Biocompatibility and low autofluorescence
The printing material is non-cytotoxic according to ISO 10993-5. This makes IP-Visio suitable for cell-friendly 3D scaffolds. With this material, high-precision microstructures can be fabricated to mimic realistic and high-precision microenvironments. An exemplary application is multi-cell scaffolds that serve as supporting material to seed and study cells in 3D.
Moreover, IP-Visio shows a very low autofluorescence. This property allows a clear view through the printed scaffolds. Scientists can analyze cellular components and processes by means of fluorescence microscopy without interference of the printed structures.
Versatile 3D Microfabrication enhanced for life sciences
Nanoscribe’s 3D printing technology give rise to numerous ways to produce 3D microenvironments that resemble the natural surrounding conditions of cells known in the human body. The technology enables the direct fabrication of intricate 3D microstructures with outstanding shape accuracy, resulting in pioneering work in 3D cell scaffolds. Research results have been published, for example, on retinal tissue engineering, cancer research, and the first 3D-printed blood-brain barrier model for drug screening.
At SPIE BiOS (booth 8256) and Photonics West (hall E, Booth 3254) Nanoscribe will present the printing material with 3D-printed microstructures.
Img. 1: Checkerboard-like structures printed with IP-Visio. Left: The structures exhibit a red-fluorescent signal due to a more pronounced Fibronectin protein adsorption compared to the glass surface. Right: IP-Visio’s low fluorescence allows a clear view of the inner cell proteins. Images: S. Bertels, M. Bastmeyer, KIT
Img. 2: Biocompatible 3D microstructure fabricated with Nanoscribe’s printing technology
Img. 3: IP-Visio: Nanoscribe’s new printing material for life science applications
Img. 4: Nanoscribe’s Photonic Professional GT2 3D printer produces filigree structures of nearly any 3D shape